A screen of chemical modifications identifies position-specific modification by UNA to most potently reduce siRNA off-target effects

Small interfering RNAs (siRNAs) are now established as the preferred tool to inhibit gene function in mammalian cells yet trigger unintended gene silencing due to their inherent miRNA-like behavior. Such off-target effects are primarily mediated by the sequence-specific interaction between the siRNA...

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Auteurs principaux: Bramsen, Jesper B., Pakula, Malgorzata M., Hansen, Thomas B., Bus, Claus, Langkjær, Niels, Odadzic, Dalibor, Smicius, Romualdas, Wengel, Suzy L., Chattopadhyaya, Jyoti, Engels, Joachim W., Herdewijn, Piet, Wengel, Jesper, Kjems, Jørgen
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 2010
Sujets:
Molecular Biology
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2943616/
https://ncbi.nlm.nih.gov/pubmed/20453030
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq341
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