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High Resolution Mapping of Protein Sequence–Function Relationships
We present a large-scale approach to investigate the functional consequences of sequence variation in a protein. The approach entails the display of hundreds of thousands of protein variants, moderate selection for activity, and high throughput DNA sequencing to quantify the performance of each vari...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2938879/ https://ncbi.nlm.nih.gov/pubmed/20711194 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1492 |
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