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High-Throughput Genotyping of Single Nucleotide Polymorphisms in the Plasmodium falciparum dhfr Gene by Asymmetric PCR and Melt-Curve Analysis

Mutations within the Plasmodium falciparum dihydrofolate reductase gene (Pfdhfr) contribute to resistance to antimalarials such as sulfadoxine-pyrimethamine (SP). Of particular importance are the single nucleotide polymorphisms (SNPs) within codons 51, 59, 108, and 164 in the Pfdhfr gene that are as...

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Detalhes bibliográficos
Main Authors: Cruz, Rochelle E., Shokoples, Sandra E., Manage, Dammika P., Yanow, Stephanie K.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2937747/
https://ncbi.nlm.nih.gov/pubmed/20631115
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00634-10
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