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Detection and Genotyping of Oocysts of Cryptosporidium parvum by Real-Time PCR and Melting Curve Analysis
Several real-time PCR procedures for the detection and genotyping of oocysts of Cryptosporidium parvum were evaluated. A 40-cycle amplification of a 157-bp fragment from the C. parvum β-tubulin gene detected individual oocysts which were introduced into the reaction mixture by micromanipulation. SYB...
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| Main Authors: | , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2002
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC130769/ https://ncbi.nlm.nih.gov/pubmed/12202559 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.40.9.3237-3244.2002 |
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