Detection and Genotyping of Oocysts of Cryptosporidium parvum by Real-Time PCR and Melting Curve Analysis

Several real-time PCR procedures for the detection and genotyping of oocysts of Cryptosporidium parvum were evaluated. A 40-cycle amplification of a 157-bp fragment from the C. parvum β-tubulin gene detected individual oocysts which were introduced into the reaction mixture by micromanipulation. SYB...

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Autori principali: Tanrıverdi, Sultan, Tanyeli, Atila, Başlamışlı, Fikri, Köksal, Fatih, Kılınç, Yurdanur, Feng, Xiaochuan, Batzer, Glenda, Tzipori, Saul, Widmer, Giovanni
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2002
Soggetti:
Parasitology
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC130769/
https://ncbi.nlm.nih.gov/pubmed/12202559
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.40.9.3237-3244.2002
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