Detection and Genotyping of Oocysts of Cryptosporidium parvum by Real-Time PCR and Melting Curve Analysis

Several real-time PCR procedures for the detection and genotyping of oocysts of Cryptosporidium parvum were evaluated. A 40-cycle amplification of a 157-bp fragment from the C. parvum β-tubulin gene detected individual oocysts which were introduced into the reaction mixture by micromanipulation. SYB...

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הוצא לאור ב:	J Clin Microbiol
Principais autores:	Tanrıverdi, Sultan, Tanyeli, Atila, Başlamışlı, Fikri, Köksal, Fatih, Kılınç, Yurdanur, Feng, Xiaochuan, Batzer, Glenda, Tzipori, Saul, Widmer, Giovanni
פורמט:	Artigo
שפה:	Inglês
יצא לאור:	American Society for Microbiology (ASM) 2002
נושאים:	Parasitology
גישה מקוונת:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC130769/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12202559/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.40.9.3237-3244.2002
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