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Multiplexed Real-Time PCR Assay for Discrimination of Plasmodium Species with Improved Sensitivity for Mixed Infections

The implementation of real-time PCR for the diagnosis of malaria has been hampered by poor sensitivity for the detection of mixed infections. We have optimized a method that enhances the sensitivity of detection of minor species in mixed infections within a single multiplex reaction. Our assay uses...

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Hlavní autoři: Shokoples, Sandra E., Ndao, Momar, Kowalewska-Grochowska, Kinga, Yanow, Stephanie K.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology (ASM) 2009
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2668309/
https://ncbi.nlm.nih.gov/pubmed/19244467
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01858-08
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