Pyrosequencing, a High-Throughput Method for Detecting Single Nucleotide Polymorphisms in the Dihydrofolate Reductase and Dihydropteroate Synthetase Genes of Plasmodium falciparum

A pyrosequencing protocol was developed as a rapid and reliable method to identify the mutations of the dhfr and dhps genes of Plasmodium falciparum that are associated with antifolate resistance. The accuracy and specificity of this method were tested using six laboratory-cultured P. falciparum iso...

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Detalhes bibliográficos
Main Authors: Zhou, Zhiyong, Poe, Amanda C., Limor, Josef, Grady, Katharine K., Goldman, Ira, McCollum, Andrea M., Escalante, Ananias A., Barnwell, John W., Udhayakumar, Venkatachalam
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2006
Assuntos:
Parasitology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1698350/
https://ncbi.nlm.nih.gov/pubmed/16957045
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01209-06
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