Pyrosequencing, a High-Throughput Method for Detecting Single Nucleotide Polymorphisms in the Dihydrofolate Reductase and Dihydropteroate Synthetase Genes of Plasmodium falciparum

A pyrosequencing protocol was developed as a rapid and reliable method to identify the mutations of the dhfr and dhps genes of Plasmodium falciparum that are associated with antifolate resistance. The accuracy and specificity of this method were tested using six laboratory-cultured P. falciparum iso...

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Autors principals: Zhou, Zhiyong, Poe, Amanda C., Limor, Josef, Grady, Katharine K., Goldman, Ira, McCollum, Andrea M., Escalante, Ananias A., Barnwell, John W., Udhayakumar, Venkatachalam
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2006
Matèries:
Parasitology
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1698350/
https://ncbi.nlm.nih.gov/pubmed/16957045
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01209-06
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