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Obstacles of Multiplex Real-Time PCR for Bacterial 16S rDNA: Primer Specifity and DNA Decontamination of Taq Polymerase

BACKGROUND: The detection of a broad range of bacteria by PCR is applied for the screening of blood and blood products with special attention to platelet concentrates. For practical use it is desirable that detection systems include Gram-positive, Gram-negative and non-Gram-stainable bacteria. It is...

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Autors principals: Philipp, Sebastian, Huemer, Hartwig P., Irschick, Eveline U., Gassner, Christoph
Format: Artigo
Idioma:Inglês
Publicat: S. Karger AG 2010
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2914405/
https://ncbi.nlm.nih.gov/pubmed/20737013
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1159/000265571
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