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Incomplete primer extension during in vitro DNA amplification catalyzed by Taq polymerase; exploitation for DNA sequencing.
Polyacrylamide gel electrophoresis of DNA fragments obtained by the polymerase chain reaction using Taq polymerase revealed the presence of multiple fragments shorter than the expected product. These abortive extension products were observed even when analysis by agarose gel electrophoresis showed o...
保存先:
| 出版年: | Nucleic Acids Res |
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| 主要な著者: | , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
1989
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| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC335201/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2602138/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.23.9613 |
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