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Incomplete primer extension during in vitro DNA amplification catalyzed by Taq polymerase; exploitation for DNA sequencing.

Polyacrylamide gel electrophoresis of DNA fragments obtained by the polymerase chain reaction using Taq polymerase revealed the presence of multiple fragments shorter than the expected product. These abortive extension products were observed even when analysis by agarose gel electrophoresis showed o...

詳細記述

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書誌詳細
出版年:Nucleic Acids Res
主要な著者: Olsen, D B, Eckstein, F
フォーマット: Artigo
言語:Inglês
出版事項: Oxford University Press 1989
オンライン・アクセス:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC335201/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2602138/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.23.9613
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