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Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

We describe a method for observing real time replication of individual DNA molecules mediated by proteins of the bacteriophage replication system. Linearized λ DNA is modified to have a biotin on the end of one strand, and a digoxigenin moiety on the other end of the same strand. The biotinylated en...

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Bibliografische gegevens
Hoofdauteurs: Kulczyk, Arkadiusz W., Tanner, Nathan A., Loparo, Joseph J., Richardson, Charles C., van Oijen, Antoine M.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: MyJove Corporation 2010
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2913797/
https://ncbi.nlm.nih.gov/pubmed/20332766
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/1689
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