Real-time single-molecule observation of rolling-circle DNA replication

We present a simple technique for visualizing replication of individual DNA molecules in real time. By attaching a rolling-circle substrate to a TIRF microscope-mounted flow chamber, we are able to monitor the progression of single-DNA synthesis events and accurately measure rates and processivities...

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Hlavní autoři: Tanner, Nathan A., Loparo, Joseph J., Hamdan, Samir M., Jergic, Slobodan, Dixon, Nicholas E., van Oijen, Antoine M.
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2009
Témata:
Methods Online
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2651787/
https://ncbi.nlm.nih.gov/pubmed/19155275
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp006
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