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Real-time single-molecule observation of rolling-circle DNA replication
We present a simple technique for visualizing replication of individual DNA molecules in real time. By attaching a rolling-circle substrate to a TIRF microscope-mounted flow chamber, we are able to monitor the progression of single-DNA synthesis events and accurately measure rates and processivities...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
2009
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2651787/ https://ncbi.nlm.nih.gov/pubmed/19155275 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp006 |
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