Optimizing Methods to Recover Absolute FRET Efficiency from Immobilized Single Molecules

Microscopy-based fluorescence resonance energy transfer (FRET) experiments measure donor and acceptor intensities by isolating these signals with a series of optical elements. Because this filtering discards portions of the spectrum, the observed FRET efficiency is dependent on the set of filters in...

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Bibliographic Details
Main Authors: McCann, James J., Choi, Ucheor B., Zheng, Liqiang, Weninger, Keith, Bowen, Mark E.
Format: Artigo
Language:Inglês
Published: The Biophysical Society 2010
Subjects:
Spectroscopy, Imaging, and Other Techniques
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2913196/
https://ncbi.nlm.nih.gov/pubmed/20682275
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2010.04.063
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