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Optimizing Methods to Recover Absolute FRET Efficiency from Immobilized Single Molecules
Microscopy-based fluorescence resonance energy transfer (FRET) experiments measure donor and acceptor intensities by isolating these signals with a series of optical elements. Because this filtering discards portions of the spectrum, the observed FRET efficiency is dependent on the set of filters in...
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| Main Authors: | , , , , |
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| Formáid: | Artigo |
| Teanga: | Inglês |
| Foilsithe: |
The Biophysical Society
2010
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| Ábhair: | |
| Rochtain Ar Líne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2913196/ https://ncbi.nlm.nih.gov/pubmed/20682275 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2010.04.063 |
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