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Immobilization of Proteins for Single-Molecule Fluorescence Resonance Energy Transfer Measurements of Conformation and Dynamics

Fluorescence resonance energy transfer provides information about protein structure and dynamics. Single-molecule analysis can capture the information normally lost through ensemble averaging of heterogeneous and dynamic samples. Immobilization of single molecules, under conditions that retain their...

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Detalhes bibliográficos
Publicado no:Methods Mol Biol
Main Authors: Choi, Ucheor B., Weninger, Keith R., Bowen, Mark E.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6081745/
https://ncbi.nlm.nih.gov/pubmed/22821514
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4614-3704-8_1
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