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Ensemble and single-molecule fluorescence-based assays to monitor DNA binding, translocation, and unwinding by iron-sulfur cluster containing helicases

Many quantitative approaches for analysis of helicase-nucleic acid interactions require a robust and specific signal, which reports on the presence of the helicase and its position on a nucleic acid lattice. Since 2006, iron-sulfur (FeS) clusters have been found in a number of helicases. They serve...

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Библиографические подробности
Главные авторы: Pugh, Robert A., Honda, Masayoshi, Spies, Maria
Формат: Artigo
Язык:Inglês
Опубликовано: 2010
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC2911022/
https://ncbi.nlm.nih.gov/pubmed/20167274
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2010.02.014
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