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Asp to Asn substitution at the first position of the DxD TOPRIM motif of recombinant bacterial topoisomerase I is extremely lethal to E. coli

The TOPRIM domain found in many nucleotidyl transferases contains a DxD motif involved in magnesium ion coordination for catalysis. Medium to high copy number plasmid clones of Yersinia pestis topoisomerase I (YpTOP) with Asp to Asn substitution at the first aspartate residue (D117N) of this motif c...

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Detalhes bibliográficos
Main Authors: Cheng, Bokun, Annamalai, Thirunavakkarasu, Sorokin, Elena, Abrenica, Maria, Aedo, Sandra, Tse-Dinh, Yuk-Ching
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2905861/
https://ncbi.nlm.nih.gov/pubmed/19013470
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2008.10.073
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