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Site-directed mutagenesis in Arabidopsis using custom-designed zinc finger nucleases

Site-directed mutagenesis in higher plants remains a significant technical challenge for basic research and molecular breeding. Here, we demonstrate targeted-gene inactivation for an endogenous gene in Arabidopsis using zinc finger nucleases (ZFNs). Engineered ZFNs for a stress-response regulator, t...

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Bibliographic Details
Main Authors: Osakabe, Keishi, Osakabe, Yuriko, Toki, Seiichi
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 2010
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2900650/
https://ncbi.nlm.nih.gov/pubmed/20508151
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1000234107
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