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Optimisation of chemical protein cleavage for erythropoietin semi-synthesis using native chemical ligation
Selective protein cleavage at methionine residues is a useful method for the production of bacterially derived protein fragments containing an N-terminal cysteine residue required for native chemical ligation. Here we describe an optimised procedure for cyanogen bromide-mediated protein cleavage, an...
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| Autors principals: | , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Royal Society of Chemistry
2008
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2898651/ https://ncbi.nlm.nih.gov/pubmed/18931805 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/b811501j |
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