Combined Use of Fluorescent Dyes and Flow Cytometry To Quantify the Physiological State of Pichia pastoris during the Production of Heterologous Proteins in High-Cell-Density Fed-Batch Cultures

Matching both the construction of a recombinant strain and the process design with the characteristics of the target protein has the potential to significantly enhance bioprocess performance, robustness, and reproducibility. The factors affecting the physiological state of recombinant Pichia pastori...

وصف كامل

محفوظ في:
التفاصيل البيبلوغرافية
المؤلفون الرئيسيون: Hyka, Petr, Züllig, Thomas, Ruth, Claudia, Looser, Verena, Meier, Christian, Klein, Joachim, Melzoch, Karel, Meyer, Hans-Peter, Glieder, Anton, Kovar, Karin
التنسيق: Artigo
اللغة:Inglês
منشور في: American Society for Microbiology (ASM) 2010
الموضوعات:
Biotechnology
الوصول للمادة أونلاين:https://ncbi.nlm.nih.gov/pmc/articles/PMC2897410/
https://ncbi.nlm.nih.gov/pubmed/20472737
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02475-09
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