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A radical on the Met-Tyr-Trp modification required for catalase activity in catalase-peroxidase is established by isotopic labeling and site-directed mutagenesis

[Image: see text] A transient tyrosyl-like radical with a narrow doublet X-band EPR signal is present during catalase turnover by M. tuberculosis catalase-peroxidase (KatG). Labeling of KatG with β-methylene deuterated tyrosine causes a collapse of the doublet to a singlet, while for 3,5- ring-deute...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Zhao, Xiangbo, Suarez, Javier, Khajo, Abdelahad, Yu, Shengwei, Metlitsky, Leonid, Magliozzo, Richard S.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2010
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC2897066/
https://ncbi.nlm.nih.gov/pubmed/20507091
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja103311e
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