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Site-specific Fluorescent Labeling of Poly-histidine Sequences Using a Metal-chelating Cysteine

Coupling genetically encoded target sequences with specific and selective labeling strategies has made it possible to utilize fluorescence spectroscopy in complex mixtures to investigate the structure, function, and dynamics of proteins. Thus, there is a growing need for a repertoire of such labelin...

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Detalhes bibliográficos
Main Authors: Krishnan, Beena, Szymanska, Aneta, Gierasch, Lila M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2896745/
https://ncbi.nlm.nih.gov/pubmed/17313455
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1747-0285.2007.00463.x
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