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Metal- and Affinity-Specific Dual Labeling of Cysteine-Rich Proteins for Identification of Metal-Binding Sites

[Image: see text] Here, using human metallothionein (MT2) as an example, we describe an improved strategy based on differential alkylation coupled to MS, assisted by zinc probe monitoring, for identification of cysteine-rich binding sites with nanomolar and picomolar metal affinity utilizing iodoace...

詳細記述

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書誌詳細
出版年:Anal Chem
主要な著者: Peris-Díaz, Manuel David, Guran, Roman, Zitka, Ondrej, Adam, Vojtech, Krężel, Artur
フォーマット: Artigo
言語:Inglês
出版事項: American Chemical Society 2020
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC7547867/
https://ncbi.nlm.nih.gov/pubmed/32786475
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.0c01604
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