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The amino acid sequence around the active-site cysteine and histidine residues, and the buried cysteine residues in ficin
Ficin that had been prepared from the latex of Ficus glabrata by salt fractionation and chromatography on carboxymethylcellulose was completely and irreversibly inhibited with 1,3-dibromo[2-(14)C]acetone and then treated with N-(4-dimethylamino-3,5-dinitrophenyl)maleimide in 6m-guanidinium chloride....
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1970
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1178866/ https://ncbi.nlm.nih.gov/pubmed/5420043 |
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