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The location of the active-site histidine residue in the primary sequence of papain
Papain that had been irreversibly inhibited with 1,3-dibromo[2-(14)C]acetone was reduced with sodium borohydride and carboxymethylated with iodoacetic acid. After digestion with trypsin and α-chymotrypsin the radioactive peptides were purified chromatographically. Their amino acid composition indica...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1968
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1198893/ https://ncbi.nlm.nih.gov/pubmed/5673531 |
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