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High-Yield Intra- and Extracellular Protein Production Using Bacillus megaterium
The Bacillus megaterium protein production system based on the inducible promoter of the xyl operon (P(xylA)) was systematically optimized. Multiple changes in basic promoter elements, such as the −10 and −35 region and the ribosome-binding site, resulted in an 18-fold increase of protein production...
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| 主要な著者: | , , , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
American Society for Microbiology (ASM)
2010
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2893505/ https://ncbi.nlm.nih.gov/pubmed/20435764 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00431-10 |
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