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High-Yield Intra- and Extracellular Protein Production Using Bacillus megaterium
The Bacillus megaterium protein production system based on the inducible promoter of the xyl operon (P(xylA)) was systematically optimized. Multiple changes in basic promoter elements, such as the −10 and −35 region and the ribosome-binding site, resulted in an 18-fold increase of protein production...
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| Main Authors: | , , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
2010
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2893505/ https://ncbi.nlm.nih.gov/pubmed/20435764 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00431-10 |
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