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Insights into polymer versus oligosaccharide synthesis: mutagenesis and mechanistic studies of a novel levansucrase from Bacillus megaterium

A novel levansucrase was identified in the supernatant of a cell culture of Bacillus megaterium DSM319. In order to test for the contribution of specific amino acid residues to levansucrase catalysis, the wild-type enzyme along with 16 variants based on sequence alignments and structural information...

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Bibliografiske detaljer
Main Authors: Homann, Arne, Biedendieck, Rebekka, Götze, Sven, Jahn, Dieter, Seibel, Jürgen
Format: Artigo
Sprog:Inglês
Udgivet: Portland Press Ltd. 2007
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2049016/
https://ncbi.nlm.nih.gov/pubmed/17608626
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20070600
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