A Bacillus megaterium Plasmid System for the Production, Export, and One-Step Purification of Affinity-Tagged Heterologous Levansucrase from Growth Medium

A multiple vector system for the production and export of recombinant affinity-tagged proteins in Bacillus megaterium was developed. Up to 1 mg/liter of a His(6)-tagged or Strep-tagged Lactobacillus reuteri levansucrase was directed into the growth medium, using the B. megaterium esterase LipA signa...

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Bibliografiska uppgifter
Huvudupphovsmän: Malten, Marco, Biedendieck, Rebekka, Gamer, Martin, Drews, Ann-Christin, Stammen, Simon, Buchholz, Klaus, Dijkhuizen, Lubbert, Jahn, Dieter
Materialtyp: Artigo
Språk:Inglês
Publicerad: American Society for Microbiology 2006
Ämnen:
Genetics and Molecular Biology
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC1392972/
https://ncbi.nlm.nih.gov/pubmed/16461726
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.72.2.1677-1679.2006
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