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Probing DNA Binding, DNA Opening and Assembly of a Downstream Clamp/Jaw in E. coli RNA Polymerase – λP(R) Promoter Complexes Using Salt and the Physiological Anion Glutamate
Transcription by all RNA polymerases (RNAP) requires a series of large-scale conformational changes to form the transcriptionally-competent open complex RP(o). At the λP(R) promoter, E. coli σ(70) RNAP first forms a wrapped, closed 100 bp complex I(1). The subsequent step opens the entire 13 base DN...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2010
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2893406/ https://ncbi.nlm.nih.gov/pubmed/20201585 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi100092a |
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