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Increasing the conformational entropy of the Ω-loop lid domain in PEPCK impairs catalysis and decreases catalytic fidelity

Many studies have shown that the dynamic motions of individual protein segments can play an important role in enzyme function. Recent structural studies on the gluconeogenic enzyme PEPCK demonstrate that PEPCK contains a 10-residue Ω-loop domain that acts as an active site lid. Based upon these stru...

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Autori principali: Johnson, Troy A., Holyoak, Todd
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2010
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2893389/
https://ncbi.nlm.nih.gov/pubmed/20476774
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi100399e
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