A Distal Point Mutation in the Streptavidin-biotin Complex Preserves Structure but Diminishes Binding Affinity: Experimental Evidence for Electronic Polarization Effects?

We have identified a distal point mutation in streptavidin that causes a 1000-fold reduction in biotin binding affinity without disrupting the equilibrium complex structure. The F130L mutation creates a small cavity occupied by a water molecule, but all neighboring side chain positions are preserved...

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Hauptverfasser: Baugh, Loren, Le Trong, Isolde, Cerutti, David S., Gülich, Susanne, Stayton, Patrick S., Stenkamp, Ronald E., Lybrand, Terry P.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2010
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2885148/
https://ncbi.nlm.nih.gov/pubmed/20462252
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi1005392
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