Cloning immunoglobulin variable domains for expression by the polymerase chain reaction.
We have designed a set of oligonucleotide primers to amplify the cDNA of mouse immunoglobulin heavy and light chain variable domains by the polymerase chain reaction. The primers incorporate restriction sites that allow the cDNA of the variable domains to be force-cloned for sequencing and expressio...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1989
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC287235/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2726754/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.10.3833 |
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