Cloning immunoglobulin variable domains for expression by the polymerase chain reaction.

We have designed a set of oligonucleotide primers to amplify the cDNA of mouse immunoglobulin heavy and light chain variable domains by the polymerase chain reaction. The primers incorporate restriction sites that allow the cDNA of the variable domains to be force-cloned for sequencing and expressio...

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Veröffentlicht in:	Proc Natl Acad Sci U S A
Hauptverfasser:	Orlandi, R, Güssow, D H, Jones, P T, Winter, G
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	National Academy of Sciences 1989
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC287235/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2726754/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.10.3833
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