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Cloning immunoglobulin variable domains for expression by the polymerase chain reaction.

We have designed a set of oligonucleotide primers to amplify the cDNA of mouse immunoglobulin heavy and light chain variable domains by the polymerase chain reaction. The primers incorporate restriction sites that allow the cDNA of the variable domains to be force-cloned for sequencing and expressio...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Orlandi, R, Güssow, D H, Jones, P T, Winter, G
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1989
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC287235/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2726754/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.10.3833
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