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Allele-specific enzymatic amplification of beta-globin genomic DNA for diagnosis of sickle cell anemia.

A rapid nonradioactive approach to the diagnosis of sickle cell anemia is described based on an allele-specific polymerase chain reaction (ASPCR). This method allows direct detection of the normal or the sickle cell beta-globin allele in genomic DNA without additional steps of probe hybridization, l...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Wu, D Y, Ugozzoli, L, Pal, B K, Wallace, R B
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1989
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC286997/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2704745/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.8.2757
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