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Allele-specific enzymatic amplification of beta-globin genomic DNA for diagnosis of sickle cell anemia.
A rapid nonradioactive approach to the diagnosis of sickle cell anemia is described based on an allele-specific polymerase chain reaction (ASPCR). This method allows direct detection of the normal or the sickle cell beta-globin allele in genomic DNA without additional steps of probe hybridization, l...
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| Publié dans: | Proc Natl Acad Sci U S A |
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| Auteurs principaux: | , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
1989
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC286997/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2704745/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.8.2757 |
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