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Allele-specific enzymatic amplification of beta-globin genomic DNA for diagnosis of sickle cell anemia.

A rapid nonradioactive approach to the diagnosis of sickle cell anemia is described based on an allele-specific polymerase chain reaction (ASPCR). This method allows direct detection of the normal or the sickle cell beta-globin allele in genomic DNA without additional steps of probe hybridization, l...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Wu, D Y, Ugozzoli, L, Pal, B K, Wallace, R B
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1989
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC286997/
https://ncbi.nlm.nih.gov/pubmed/2704745
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