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Tuning the pK(a) of Fluorescein to Optimize Binding Assays
The phenolic pK(a) of fluorescein varies depending on its environment. The fluorescence of the dye varies likewise. Accordingly, a change in fluorescence can report on the association of a fluorescein-conjugate to another molecule. Here, we demonstrate how to optimize this process with chemical synt...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2868592/ https://ncbi.nlm.nih.gov/pubmed/17672523 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac070907g |
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