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Detection and Isolation of Viable Mouse IL-17-Secreting T Cells
The MACS Cytokine Secretion Assay technology allows detection of secreted cytokines on the single cell level and sensitive isolation of viable cytokine-secreting cells. In order to label IL-17-secreting cells, a single cell suspension of mouse splenocytes is prepared and stimulated at 37°C with PMA/...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2865976/ https://ncbi.nlm.nih.gov/pubmed/19229172 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/1037 |
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