Detection and Isolation of Viable Mouse IL-17-Secreting T Cells

The MACS Cytokine Secretion Assay technology allows detection of secreted cytokines on the single cell level and sensitive isolation of viable cytokine-secreting cells. In order to label IL-17-secreting cells, a single cell suspension of mouse splenocytes is prepared and stimulated at 37°C with PMA/...

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Bibliographische Detailangaben
Hauptverfasser: Foerster, Anna, Assenmacher, Mario, Niemoeller, Michaela, Rankin, Elly, Mohaupt, Mariette, Richter, Anne
Format: Artigo
Sprache:Inglês
Veröffentlicht: MyJove Corporation 2008
Schlagworte:
Immunology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2865976/
https://ncbi.nlm.nih.gov/pubmed/19229172
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/1037
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