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Transition states of uncatalyzed hydrolysis and aminolysis reactions of a ribosomal P-site substrate determined by kinetic isotope effects

The ester bond of peptidyl-tRNA undergoes nucleophilic attack both in solution and catalyzed by the ribosome. To characterize the uncatalyzed hydrolysis reaction - a model of peptide release - the transition state structure for hydrolysis of a peptidyl-tRNA mimic was determined. Kinetic isotope effe...

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Main Authors: Hiller, David A, Zhong, Minghong, Singh, Vipender, Strobel, Scott A
Format: Artigo
Jezik:Inglês
Izdano: 2010
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2864349/
https://ncbi.nlm.nih.gov/pubmed/20359191
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi901458x
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