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Kinetic isotope effects in Ras-catalyzed GTP hydrolysis: Evidence for a loose transition state
A remote labeling method has been developed to determine (18)O kinetic isotope effects (KIEs) in Ras-catalyzed GTP hydrolysis. Substrate mixtures consist of (13)C-depleted GTP and [(18)O,(13)C]GTP that contains (18)O at phosphoryl positions of mechanistic interest and (13)C at all carbon positions o...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC428437/ https://ncbi.nlm.nih.gov/pubmed/15178760 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0401675101 |
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