Probing the catalytic roles of Arg548 and Gln552 in the carboxyl-transferase domain of the Rhizobium etli pyruvate carboxylase by site-directed mutagenesis

The roles of Arg548 and Gln552 residues in the active site of the carboxyl transferase domain of Rhizobium etli pyruvate carboxylase were investigated using site-directed mutagenesis. Mutation of Arg548 to alanine or glutamine resulted in the destabilisation of the quaternary structure of the enzyme...

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Hlavní autoři: Duangpan, Saowapa, Jitrapakdee, Sarawut, Adina-Zada, Abdussalam, Byrne, Lindsay, Zeczycki, Tonya N., St. Maurice, Martin, Cleland, W.Wallace, Wallace, John C., Attwood, Paul V.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2010
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2864305/
https://ncbi.nlm.nih.gov/pubmed/20230056
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi901894t
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