Escherichia coli MW005: lambda Red-mediated recombineering and copy-number induction of oriV-equipped constructs in a single host

BACKGROUND: Escherichia coli strain EL350 contains chromosomally integrated phage lambda Red recombinase genes enabling this strain to be used for modifying the sequence of resident clones via recombineering. BAC and fosmid clones are highly suitable for modification by recombineering but, because t...

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Bibliographic Details
Main Authors: Westenberg, Marcel, Bamps, Sophie, Soedling, Helen, Hope, Ian A, Dolphin, Colin T
Format: Artigo
Language:Inglês
Published: BioMed Central 2010
Subjects:
Methodology article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2864197/
https://ncbi.nlm.nih.gov/pubmed/20350301
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-10-27
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