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A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones

BACKGROUND: Recombineering is a genetic engineering tool that enables facile modification of large episomal clones, e.g. BACs, fosmids. We have previously adapted this technology to generate, directly from fosmid-based genomic clones, fusion gene reporter constructs designed to investigate gene expr...

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Detalhes bibliográficos
Main Authors: Hirani, Nisha, Westenberg, Marcel, Gami, Minaxi S, Davis, Paul, Hope, Ian A, Dolphin, Colin T
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3561212/
https://ncbi.nlm.nih.gov/pubmed/23281894
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-13-1
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