Use of a High-Resolution Melt Assay To Characterize Codon 54 of the cyp51A Gene of Aspergillus fumigatus on a Rotor-Gene 6000 Instrument

A high-resolution melt (HRM) assay using a Rotor-Gene 6000 instrument was developed to characterize the codon for glycine 54 in the cyp51A genes from 13 reference isolates and 12 clinical isolates of Aspergillus fumigatus. Mutations in this codon confer reduced susceptibility to itraconazole and pos...

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Detalhes bibliográficos
Main Authors: Tuohy, M. J., Reja, V., Park, S., Perlin, D. S., Wnek, M., Procop, G. W., Yen-Lieberman, B.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2010
Assuntos:
Susceptibility
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2863670/
https://ncbi.nlm.nih.gov/pubmed/20194699
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.01712-09
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