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Single-Pair Fluorescence Resonance Energy Transfer (spFRET) for the High Sensitivity Analysis of Low-Abundance Proteins Using Aptamers as Molecular Recognition Elements

We have developed a strategy for the detection of single protein molecules, which uses single-pair fluorescence resonance energy transfer (spFRET) as the readout modality and provides exquisite analytical sensitivity and reduced assay turn-around-time by eliminating various sample pre-processing ste...

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Detalhes bibliográficos
Main Authors: Lee, Wonbae, Obubuafo, Anne, Lee, Yong-Ill, Davis, Lloyd M., Soper, Steven A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2863098/
https://ncbi.nlm.nih.gov/pubmed/19802688
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10895-009-0540-5
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