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A Novel Immobilization Method for Single Protein spFRET Studies

We have developed a new method for immobilization of single proteins by utilizing streptavidin-biotin and protein L-antibody interactions on glass coverslips coated with polyethylene glycol. The method is particularly well suited for single-molecule fluorescence studies. A monomeric, detergent-solub...

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Detalhes bibliográficos
Main Authors: Pal, Prithwish, Lesoine, John F., Lieb, M. Andreas, Novotny, Lukas, Knauf, Philip A.
Formato: Artigo
Idioma:Inglês
Publicado em: Biophysical Society 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1366648/
https://ncbi.nlm.nih.gov/pubmed/15908583
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.062794
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