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A Novel Immobilization Method for Single Protein spFRET Studies
We have developed a new method for immobilization of single proteins by utilizing streptavidin-biotin and protein L-antibody interactions on glass coverslips coated with polyethylene glycol. The method is particularly well suited for single-molecule fluorescence studies. A monomeric, detergent-solub...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Biophysical Society
2005
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1366648/ https://ncbi.nlm.nih.gov/pubmed/15908583 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.062794 |
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