Real-time observation of the conformational dynamics of mitochondrial Hsp70 by spFRET

The numerous functions of the important class of molecular chaperones, heat shock proteins 70 (Hsp70), rely on cycles of intricate conformational changes driven by ATP-hydrolysis and regulated by cochaperones and substrates. Here, we used Förster resonance energy transfer to study the conformational...

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Hauptverfasser: Sikor, Martin, Mapa, Koyeli, von Voithenberg, Lena Voith, Mokranjac, Dejana, Lamb, Don C
Format: Artigo
Sprache:Inglês
Veröffentlicht: European Molecular Biology Organization 2013
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3671257/
https://ncbi.nlm.nih.gov/pubmed/23624933
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/emboj.2013.89
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