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Real-time observation of the conformational dynamics of mitochondrial Hsp70 by spFRET

The numerous functions of the important class of molecular chaperones, heat shock proteins 70 (Hsp70), rely on cycles of intricate conformational changes driven by ATP-hydrolysis and regulated by cochaperones and substrates. Here, we used Förster resonance energy transfer to study the conformational...

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Detalhes bibliográficos
Main Authors: Sikor, Martin, Mapa, Koyeli, von Voithenberg, Lena Voith, Mokranjac, Dejana, Lamb, Don C
Formato: Artigo
Idioma:Inglês
Publicado em: European Molecular Biology Organization 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3671257/
https://ncbi.nlm.nih.gov/pubmed/23624933
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/emboj.2013.89
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