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Urea-Induced Unfolding of the Immunity Protein Im9 Monitored by spFRET

We have studied the urea-induced unfolding of the E colicin immunity protein Im9 using diffusion single-pair fluorescence resonance energy transfer. Detailed examination of the proximity ratio of the native and denatured molecules over a wide range of urea concentrations suggests that the conformati...

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Detalhes bibliográficos
Main Authors: Tezuka-Kawakami, Tomoko, Gell, Chris, Brockwell, David J., Radford, Sheena E., Smith, D. Alastair
Formato: Artigo
Idioma:Inglês
Publicado em: Biophysical Society 2006
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1544283/
https://ncbi.nlm.nih.gov/pubmed/16798813
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.088344
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