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Mass Spectral Characterization of Organophosphate-Labeled, Tyrosine-Containing Peptides: Characteristic Mass Fragments and A New Binding Motif for Organophosphates
We have identified organophosphorus agent (OP)-tyrosine adducts on 12 different proteins labeled with 6 different OP. Labeling was achieved by treating pure proteins with up to 40-fold molar excess of OP at pH 8–8.6. OP-treated proteins were digested with trypsin, and peptides were separated by HPLC...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2860652/ https://ncbi.nlm.nih.gov/pubmed/19762289 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jchromb.2009.07.026 |
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